Daun dan Kulit Buah Kopi Robusta (Coffea canephora Pierre ex A.Froehner) Sebagai Sumber Senyawa Bioaktif untuk Aplikasi Farmakologi
DOI:
https://doi.org/10.35334/fp1sg371Keywords:
Coffea canephora, fitofarmaka, metabolit sekunderAbstract
Kopi menjadi salah satu komoditas perkebunan strategis dengan nilai ekonomi tinggi dan distribusi konsumsi yang luas secara global. Selain biji kopi yang menjadi produk utama, bagian tanaman lain seperti daun dan kulit buah kopi diketahui mengandung beragam senyawa metabolit sekunder yang berpotensi memberikan manfaat farmakologis. Penelitian ini bertujuan untuk mengidentifikasi kandungan metabolit sekunder pada ekstrak daun dan kulit buah kopi robusta melalui uji fitokimia kualitatif. Sampel diambil dari perkebunan kopi di Lampung Barat, diidentifikasi secara morfologi, kemudian diekstraksi dan dianalisis menggunakan pereaksi spesifik untuk mendeteksi alkaloid, flavonoid, fenolik/tanin, saponin, terpenoid, dan steroid. Hasil analisis menunjukkan bahwa ekstrak daun maupun kulit buah kopi robusta positif mengandung alkaloid (presipitasi putih-cokelat), flavonoid (warna kuning kecokelatan disertai busa), fenolik/tanin (warna hijau kebiruan), saponin (buih stabil ±30 detik), dan terpenoid (cincin merah), sedangkan senyawa golongan steroid tidak terdeteksi. Keberadaan senyawa tersebut mengindikasikan potensi aktivitas biologis seperti antioksidan, antiinflamasi, antimikroba, dan kardioprotektif, sebagaimana dilaporkan dalam penelitian terdahulu dalam satu dekade terakhir. Temuan ini memperkuat prospek pemanfaatan bagian non-biji kopi robusta sebagai sumber senyawa bioaktif yang dapat dikembangkan dalam industri fitofarmaka dan pangan fungsional berbasis tanaman.
References
Abeywickrama, G., Debnath, S., & Jayasena, V. (2022). Bioactive compounds and antioxidant potential of coffee pulp: A review. Journal of Food Science and Technology, 59, 353–362. https://doi.org/10.1007/s13197-021-05000-y
Ahriyasna, N. N. P., Pratiwi, I. G. A. A. E., & Putra, I. M. W. A. (2024). Antidiabetic effect of robusta coffee leaf (Coffea canephora) decoction in alloxan-induced diabetic rats. Journal of Pharmacy and Pharmacology Research, 2(1), 45–53.
APG II. (2003). An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants. Botanical Journal of the Linnean Society, 141, 399–436.
Balasundram, N., Sundram, K., & Samman, S. (2006). Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chemistry, 99(1), 191–203. https://doi.org/10.1016/j.foodchem.2005.07.042
Budijanto, S., Hidayat, M., & Setyopratomo, P. (2021). Bioactive compounds in robusta coffee pulp for functional food ingredients. International Journal on Advanced Science, Engineering and Information Technology, 11(3), 1133–1139. https://doi.org/10.18517/ijaseit.11.3.13438
Chen, X., Zhang, H., Wang, J., & Wang, C. (2020). Chemical composition and antioxidant activities of coffee leaves. Food Chemistry, 331, 127312. https://doi.org/10.1016/j.foodchem.2020.127312
Choirunnisa, N., Rahayu, S., & Sutrisno, B. (2024). Protective effect of robusta coffee pulp extract on intestinal tissue of lead acetate-induced rats. Indonesian Journal of Pharmacology, 15(2), 112–120.
Clifford, M. N., Zheng, W., Kuhnert, N., & van der Hooft, J. J. J. (2017). Chlorogenic acids and the acyl-quinic acids: Discovery, biosynthesis, and dietary roles. Journal of Agricultural and Food Chemistry, 65(33), 7098–7116. https://doi.org/10.1021/acs.jafc.7b03539
Cronquist, A. (1981). An integrated system of classification of flowering plants. Columbia University Press.
Defri, H., Ismail, R., & Yuliani, N. (2021). Antioxidant activity of Kawa Daun robusta coffee (Coffea canephora) from West Sumatra: In vitro and in silico studies. Journal of Natural Products and Resources, 7(3), 99–106.
Dias, D. R., Schwan, R. F., & Silva, J. B. (2021). Coffee pulp and husk: By-products with potential for functional food development. Trends in Food Science & Technology, 116, 317–325. https://doi.org/10.1016/j.tifs.2021.07.024
Farah, A., & Donangelo, C. M. (2006). Phenolic compounds in coffee. Brazilian Journal of Plant Physiology, 18(1), 23–36. https://doi.org/10.1590/S1677-04202006000100003
Fitriani, N., Prihantono, P., & Ramadhan, D. (2017). Phytochemical screening and antioxidant activity of Coffea canephora leaves. Journal of Tropical Pharmacy and Chemistry, 4(3), 139–145. https://doi.org/10.25026/jtpc.v4i3.152
Francis, G., Kerem, Z., Makkar, H. P. S., & Becker, K. (2002). The biological action of saponins in animal systems: A review. British Journal of Nutrition, 88(6), 587–605. https://doi.org/10.1079/BJN2002725
Gershenzon, J., & Dudareva, N. (2007). The function of terpene natural products in the natural world. Nature Chemical Biology, 3(7), 408–414. https://doi.org/10.1038/nchembio.2007.5
Grosso, G., Godos, J., Galvano, F., & Giovannucci, E. L. (2017). Coffee, caffeine, and health outcomes: An umbrella review. Annual Review of Nutrition, 37, 131–156. https://doi.org/10.1146/annurev-nutr-071816-064941
International Coffee Organization (ICO). (2022). Coffee Market Report 2021/22. https://www.ico.org
Kim, Y., Keogh, J. B., & Clifton, P. M. (2017). Benefits of phytosterols and phytostanols on lipid profiles and cardiovascular risk. Nutrition Research, 38, 1–13. https://doi.org/10.1016/j.nutres.2016.12.003
Latief, M., Nurdin, A., & Sahar, S. (2022). Antibacterial potential of robusta coffee pulp extract (Coffea canephora). Indonesian Journal of Pharmacy, 33(1), 58–64.
Martinez-Saez, N., Ullate, M., Martín-Cabrejas, M. A., Martorell, P., Genovés, S., Ramon, D., & Del Castillo, M. D. (2017). A novel antioxidant beverage for body weight control based on coffee silverskin. Food Chemistry, 225, 245–253. https://doi.org/10.1016/j.foodchem.2016.12.059
Martins, N., Barros, L., & Ferreira, I. C. F. R. (2021). Nutritional and bioactive compounds of coffee leaves: A sustainable source of functional ingredients. Food & Function, 12(1), 1–15. https://doi.org/10.1039/D0FO02555E
Muchtaridi, M., Subarnas, A., Apriyanti, E., & Yusuf, M. (2020). Phytochemical analysis and pharmacological activity of Coffea species. Journal of Applied Pharmaceutical Science, 10(6), 1–12. https://doi.org/10.7324/JAPS.2020.10601
Murthy, P. S., & Naidu, M. M. (2012). Sustainable management of coffee industry by-products and value addition—A review. Resources, Conservation and Recycling, 66, 45–58. https://doi.org/10.1016/j.resconrec.2012.06.005
Nugraha, R., Putri, N. D., & Hidayati, N. (2020). Antioxidant activity of robusta coffee leaf extract. IOP Conference Series: Earth and Environmental Science, 475, 012040. https://doi.org/10.1088/1755-1315/475/1/012040
Nugroho, D., Rahmawati, D., & Santoso, U. (2021). Caffeine content in leaves and fruit peel of robusta coffee (Coffea canephora) at different maturity stages. Asian Journal of Agriculture and Biology, 9(1), 13–20.
Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5, e47. https://doi.org/10.1017/jns.2016.41
Piironen, V., Lindsay, D. G., Miettinen, T. A., Toivo, J., & Lampi, A. M. (2000). Plant sterols: Biosynthesis, biological function and their importance to human nutrition. Journal of the Science of Food and Agriculture, 80(7), 939–966. https://doi.org/10.1002/(SICI)1097-0010(20000515)80:7<939::AID-JSFA644>3.0.CO;2-C
Rahmawati, D., Santoso, U., & Nugroho, D. (2021). Antibacterial activity of robusta coffee (Coffea canephora) fruit peel extract against Escherichia coli and Staphylococcus aureus. Biosaintifika, 13(2), 206–213. https://doi.org/10.15294/biosaintifika.v13i2.28869
Rios, M. B., Franco, M. A., Alves, A. P., et al. (2020). Chemical composition and biological activities of coffee pulp: A review. Food Research International, 137, 109409. https://doi.org/10.1016/j.foodres.2020.109409
Salminen, A., Lehtonen, M., Suuronen, T., Kaarniranta, K., & Huuskonen, J. (2008). Terpenoids: Natural inhibitors of NF-κB signaling with anti-inflammatory and anticancer potential. Cellular and Molecular Life Sciences, 65(19), 2979–2999. https://doi.org/10.1007/s00018-008-8103-5
Siregar, R. P., Hutagalung, S., & Lubis, Z. (2023). Neuroprotective effect of robusta coffee pulp extract in lead acetate-induced oxidative stress in rats. Journal of Medicinal Plants Research, 17(2), 56–63.
Sparg, S. G., Light, M. E., & van Staden, J. (2004). Biological activities and distribution of plant saponins. Journal of Ethnopharmacology, 94(2-3), 219–243. https://doi.org/10.1016/j.jep.2004.05.016
Syamsudin, S., Nurrochmad, A., & Nugroho, A. E. (2019). Cytotoxic activity of Coffea canephora leaf extract against T47D breast cancer cell lines. Indonesian Journal of Cancer Chemoprevention, 10(2), 46–51. https://doi.org/10.14499/indonesianjcanchemoprev10iss2pp46-51
Teles, S., Teles, R., & Silva, F. (2020). Terpenoids as natural preservatives in food systems. Food Research International, 136, 109482. https://doi.org/10.1016/j.foodres.2020.109482
Urbina, P., Reig, N., & Farré, M. (2018). Alkaloids: Biochemistry, ecology, and medicinal applications. Journal of Medicinal Chemistry, 61(18), 8182–8200. https://doi.org/10.1021/acs.jmedchem.8b00235
Wang, Y., Zhang, H., Zhao, Y., & Zhang, Q. (2019). Alkaloids: Therapeutic potential and mechanisms of action in microbial infection. Frontiers in Pharmacology, 10, 692. https://doi.org/10.3389/fphar.2019.00692
Wijayanti, R., Prasetyo, H., & Hidayati, L. (2018). Phagocytic activity enhancement by coffee leaf saponin extract. Pharmacognosy Journal, 10(2), 243–248. https://doi.org/10.5530/pj.2018.2.42
Wijaya, R., Nugroho, D., & Santoso, U. (2021). Sampling techniques for plant-based bioactive compound analysis. Indonesian Journal of Pharmaceutical Sciences, 12(1), 45–52.






